Institute of Microbial Technology (सूक्ष्मजीव प्रौद्योगिकी संस्थान)
A Council of Scientific & Industrial Research (वैज्ञानिक औद्योगिक अनुसंधान परिषद)
Admin Login
Back to Home
Small molecule modulators for selective autophagy from high throughput screening
Optineurin (OPTN)
The SQSTM protein also known as sequestosome-1 recognizes specific cargos such as toxic cellular waste, which then get sequestered in an autophagosome. Lack of autophagy leads to accrual of SQSTM, which is not healthy as it leads to cellular stress induced disease. Targeting SQSTM with small molecule modulators which can cause its decreased accumulation would be helpful. Indirect evidences are there for various molecules which affect the levels of SQSTM, like BRD5631 increases the SQSTM levels along with LC3II in Atg5+/+ mouse embryonic fibroblasts. On the contrary, treatment with BRD5631, BRD2716, and BRD34009 reduced levels of endogenous SQSTM in NPC1 neuronal cells suggesting complex effect of BRD series of compounds on SQSTM levels in the cellular context. Another compound clenbuterol leads to decreased levels of SQSTM in HepG2 cells and primary mouse hepatocytes. Hydroxychloroquine (HCQ) and chloroquine analog EAD1 also accumulate the SQSTM protein in lung and pancreatic cancer cell lines. Collectively all these compounds indirectly modulate the SQSTM levels in varied cell types thus can be utilized for targeting autophagy in a non-specific manner. AND (pcassay_pccompound_active[filt])&loc=s_frm
  About | Team | Contact Us | Disclaimer